FineTest
SKU:ER0033
Rat IL-10(Interleukin-10) ELISA Kit
Rat IL-10(Interleukin-10) ELISA Kit
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Product Name
Rat IL-10(Interleukin-10) ELISA Kit
Alias
IL-10 ELISA Kit, Interleukin 10 ELISA Kit, IL10 ELISA Kit, IL10A ELISA Kit, CSIF ELISA Kit, TGIF ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-10 ELISA Kit allows for the in vitro quantitative determination of IL-10 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-10 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-10 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-103 |
93 |
|
EDTA plasma(n=5) |
88-104 |
97 |
|
heparin plasma(n=5) |
91-99 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-103% |
90-101% |
87-99% |
|
EDTA plasma(n=5) |
86-100% |
84-97% |
82-91% |
|
heparin plasma(n=5) |
84-97% |
82-98% |
80-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
