FineTest
SKU:ER0018
Rat EGF(Epidermal Growth Factor) ELISA Kit
Rat EGF(Epidermal Growth Factor) ELISA Kit
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Product Name
Rat EGF(Epidermal Growth Factor) ELISA Kit
Alias
EGF ELISA Kit, URG ELISA Kit, HOMG4 ELISA Kit, Beta-Urogastrone ELISA Kit, Epidermal growth factor ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
EGF ELISA Kit allows for the in vitro quantitative determination of EGF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.906-250pg/ml
Sensitivity
< 2.344pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of EGF and the recovery rates were calculated by comparing the measured value to the expected amount of EGF in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-101 |
95 |
|
EDTA plasma(n=5) |
92-99 |
95 |
|
heparin plasma(n=5) |
90-102 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-104% |
94-103% |
85-105% |
|
EDTA plasma(n=5) |
90-101% |
87-95% |
85-94% |
|
heparin plasma(n=5) |
81-96% |
88-100% |
81-94% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
