FineTest
SKU:ER0014
Rat sCD80(Soluble Cluster of Differentiation 8) ELISA Kit
Rat sCD80(Soluble Cluster of Differentiation 8) ELISA Kit
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Product Name
Rat sCD80(Soluble Cluster of Differentiation 8) ELISA Kit
Alias
Scd80 ELISA Kit, CD80 ELISA Kit, B7-1 ELISA Kit, T-lymphocyte activation antigen CD80 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
sCD80 ELISA Kit allows for the in vitro quantitative determination of sCD80 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of sCD80 and the recovery rates were calculated by comparing the measured value to the expected amount of sCD80 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-102 |
92 |
|
EDTA plasma(n=5) |
87-103 |
95 |
|
heparin plasma(n=5) |
89-101 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sCD80 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-94% |
88-105% |
85-97% |
|
EDTA plasma(n=5) |
82-98% |
86-101% |
86-101% |
|
heparin plasma(n=5) |
83-100% |
85-94% |
87-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
