FineTest
SKU:ER0008
Rat BDNF(Brain Derived Neurotrophic Factor) ELISA Kit
Rat BDNF(Brain Derived Neurotrophic Factor) ELISA Kit
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Product Name
Rat BDNF(Brain Derived Neurotrophic Factor) ELISA Kit
Alias
BDNF ELISA Kit, Brain Derived Neurotrophic Factor ELISA Kit, ANON2 ELISA Kit, BULN2 ELISA Kit, Abrineurin ELISA Kit, neurotrophin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
BDNF ELISA Kit allows for the in vitro quantitative determination of BDNF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of BDNF and the recovery rates were calculated by comparing the measured value to the expected amount of BDNF in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-104 |
98 |
|
EDTA plasma(n=5) |
86-104 |
93 |
|
heparin plasma(n=5) |
89-105 |
100 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-105% |
85-104% |
87-105% |
|
EDTA plasma(n=5) |
83-94% |
83-97% |
84-101% |
|
heparin plasma(n=5) |
84-100% |
80-96% |
80-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
