FineTest
SKU:EM1510
Mouse His(Histamine) ELISA Kit
Mouse His(Histamine) ELISA Kit
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Product Name
Mouse His(Histamine) ELISA Kit
Alias
Histamine ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
His ELISA Kit allows for the in vitro quantitative determination of His concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100ng/ml
Sensitivity
< 0.938ng/ml
Species
Mouse
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of His and the recovery rates were calculated by comparing the measured value to the expected amount of His in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-102 |
96 |
|
EDTA plasma(n=5) |
88-104 |
96 |
|
heparin plasma(n=5) |
89-101 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of His and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-96% |
86-99% |
86-100% |
|
EDTA plasma(n=5) |
87-100% |
83-95% |
85-98% |
|
heparin plasma(n=5) |
81-88% |
80-100% |
80-86% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
