FineTest
SKU:EM1447
Mouse VIP(Vasoactive Intestinal Peptide) ELISA Kit
Mouse VIP(Vasoactive Intestinal Peptide) ELISA Kit
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Product Name
Mouse VIP(Vasoactive Intestinal Peptide) ELISA Kit
Alias
VIP ELISA Kit, vasoactive intestinal peptide ELISA Kit, VIP peptides ELISA Kit, PHM27 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
VIP ELISA Kit allows for the in vitro quantitative determination of VIP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
12.5-800pg/ml
Sensitivity
< 7.5pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of VIP and the recovery rates were calculated by comparing the measured value to the expected amount of VIP in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-103 |
95 |
|
EDTA plasma(n=5) |
88-105 |
96 |
|
heparin plasma(n=5) |
89-101 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-105% |
86-104% |
93-105% |
|
EDTA plasma(n=5) |
83-101% |
91-96% |
89-97% |
|
heparin plasma(n=5) |
85-93% |
81-93% |
83-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
