FineTest
SKU:EM1364
Mouse sIL-6R(Soluble Interleukin 6 Receptor) ELISA Kit
Mouse sIL-6R(Soluble Interleukin 6 Receptor) ELISA Kit
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Product Name
Mouse sIL-6R(Soluble Interleukin 6 Receptor) ELISA Kit
Alias
sIL-6R ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
sIL-6R ELISA Kit allows for the in vitro quantitative determination of sIL-6R concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
12.5-800pg/ml
Sensitivity
< 7.5pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of sIL-6R and the recovery rates were calculated by comparing the measured value to the expected amount of sIL-6R in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-105 |
97 |
|
EDTA plasma(n=5) |
88-102 |
92 |
|
heparin plasma(n=5) |
86-100 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sIL-6R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-97% |
93-101% |
86-99% |
|
EDTA plasma(n=5) |
82-97% |
86-95% |
85-100% |
|
heparin plasma(n=5) |
81-100% |
84-95% |
80-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
