FineTest
SKU:EM1360
Mouse SHH(Hedgehog Homolog, Sonic) ELISA Kit
Mouse SHH(Hedgehog Homolog, Sonic) ELISA Kit
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Product Name
Mouse SHH(Hedgehog Homolog, Sonic) ELISA Kit
Alias
SHH ELISA Kit, HHG1 ELISA Kit, HHG-1 ELISA Kit, HLP3 ELISA Kit, HPE3 ELISA Kit, SMMCIsonic hedgehog homolog ELISA Kit, Drosophila ELISA Kit, sonic hedgehog ELISA Kit, sonic hedgehog homolog ELISA Kit, sonic hedgehog protein ELISA Kit, TPT ELISA Kit, TPTPS ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SHH ELISA Kit allows for the in vitro quantitative determination of SHH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SHH and the recovery rates were calculated by comparing the measured value to the expected amount of SHH in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-105 |
98 |
|
EDTA plasma(n=5) |
91-102 |
99 |
|
heparin plasma(n=5) |
87-101 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SHH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-105% |
86-104% |
86-101% |
|
EDTA plasma(n=5) |
82-101% |
86-99% |
85-93% |
|
heparin plasma(n=5) |
81-97% |
80-100% |
82-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
