FineTest
SKU:EM1325
Mouse PYGM(Glycogen Phosphorylase, Muscle) ELISA Kit
Mouse PYGM(Glycogen Phosphorylase, Muscle) ELISA Kit
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Product Name
Mouse PYGM(Glycogen Phosphorylase, Muscle) ELISA Kit
Alias
PYGM ELISA Kit, Myophosphorylase ELISA Kit, Glycogen phosphorylase ELISA Kit, muscle form ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PYGM ELISA Kit allows for the in vitro quantitative determination of PYGM concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Mouse
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PYGM and the recovery rates were calculated by comparing the measured value to the expected amount of PYGM in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-100 |
92 |
|
EDTA plasma(n=5) |
93-105 |
98 |
|
heparin plasma(n=5) |
94-105 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PYGM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-105% |
88-103% |
91-100% |
|
EDTA plasma(n=5) |
83-99% |
84-100% |
83-97% |
|
heparin plasma(n=5) |
91-99% |
80-89% |
89-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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