FineTest
SKU:EM1284
Mouse PGF2α(Prostaglandin F2 Alpha) ELISA Kit
Mouse PGF2α(Prostaglandin F2 Alpha) ELISA Kit
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Product Name
Mouse PGF2α(Prostaglandin F2 Alpha) ELISA Kit
Alias
PGF2α ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
PGF2α ELISA Kit allows for the in vitro quantitative determination of PGF2α concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
7.813-500pg/ml
Sensitivity
< 4.688pg/ml
Species
Mouse
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PGF2α and the recovery rates were calculated by comparing the measured value to the expected amount of PGF2α in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
95-101 |
98 |
|
EDTA plasma(n=5) |
85-102 |
95 |
|
heparin plasma(n=5) |
88-104 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PGF2α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-101% |
86-103% |
86-102% |
|
EDTA plasma(n=5) |
82-99% |
87-97% |
85-93% |
|
heparin plasma(n=5) |
84-99% |
83-100% |
82-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
