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SKU:EM1260
Mouse PACAP-38(Pituitary Adenylate Cyclase Activating Polypeptide 38) ELISA Kit
Mouse PACAP-38(Pituitary Adenylate Cyclase Activating Polypeptide 38) ELISA Kit
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Product Name
Mouse PACAP-38(Pituitary Adenylate Cyclase Activating Polypeptide 38) ELISA Kit
Alias
PACAP-38 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PACAP-38 ELISA Kit allows for the in vitro quantitative determination of PACAP-38 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
7.813-500pg/ml
Sensitivity
< 4.688pg/ml
Species
Mouse
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PACAP-38 and the recovery rates were calculated by comparing the measured value to the expected amount of PACAP-38 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-105 |
100 |
|
EDTA plasma(n=5) |
89-103 |
96 |
|
heparin plasma(n=5) |
87-103 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PACAP-38 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-94% |
89-105% |
87-105% |
|
EDTA plasma(n=5) |
85-97% |
89-101% |
84-99% |
|
heparin plasma(n=5) |
80-93% |
87-98% |
81-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
