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EM1259

Mouse PACAP(Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit

Mouse PACAP(Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit

$450.00 USD
$450.00 USD
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size
Sensitivity
Range

Product Name

Mouse PACAP(Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit

Alias

PACAP ELISA Kit, Adcyap1 ELISA Kit, Pituitary adenylate cyclase-activating polypeptide ELISA Kit, ADCYAP1 ELISA Kit, PACAP ELISA Kit, Pituitary adenylate cyclase-activating polypeptide ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

PACAP ELISA Kit allows for the in vitro quantitative determination of PACAP concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Species

Mouse

UniProt ID

O70176

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PACAP and the recovery rates were calculated by comparing the measured value to the expected amount of PACAP in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

87-104

93

EDTA plasma(n=5)

86-100

93

heparin plasma(n=5)

91-104

98

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PACAP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

90-102%

86-105%

93-104%

EDTA plasma(n=5)

87-101%

88-101%

82-92%

heparin plasma(n=5)

83-99%

87-94%

82-96%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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