FineTest
SKU:EM1228
Mouse NE(Neutrophil Elastase) ELISA Kit
Mouse NE(Neutrophil Elastase) ELISA Kit
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Product Name
Mouse NE(Neutrophil Elastase) ELISA Kit
Alias
NE ELISA Kit, Neutrophil Elastase ELISA Kit, ELANE ELISA Kit, PMN elastase ELISA Kit, Medullasin ELISA Kit, Bone marrow serine protease ELISA Kit, Elastase-2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
NE ELISA Kit allows for the in vitro quantitative determination of NE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of NE and the recovery rates were calculated by comparing the measured value to the expected amount of NE in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-103 |
97 |
|
EDTA plasma(n=5) |
85-105 |
94 |
|
heparin plasma(n=5) |
91-105 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-105% |
87-103% |
88-104% |
|
EDTA plasma(n=5) |
90-101% |
88-101% |
85-99% |
|
heparin plasma(n=5) |
80-95% |
83-97% |
83-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
