FineTest
SKU:EM1153
Mouse IgG2a(Immunoglobulin G2a) ELISA Kit
Mouse IgG2a(Immunoglobulin G2a) ELISA Kit
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Product Name
Mouse IgG2a(Immunoglobulin G2a) ELISA Kit
Alias
IgG2a ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IgG2a ELISA Kit allows for the in vitro quantitative determination of IgG2a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.781-50ng/ml
Sensitivity
< 0.469ng/ml
Species
Mouse
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IgG2a and the recovery rates were calculated by comparing the measured value to the expected amount of IgG2a in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-101 |
94 |
|
EDTA plasma(n=5) |
87-105 |
97 |
|
heparin plasma(n=5) |
89-102 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgG2a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-101% |
87-99% |
85-105% |
|
EDTA plasma(n=5) |
82-99% |
84-100% |
82-100% |
|
heparin plasma(n=5) |
87-99% |
81-94% |
82-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
