FineTest
SKU:EM1140
Mouse IARS(Isoleucyl tRNA Synthetase) ELISA Kit
Mouse IARS(Isoleucyl tRNA Synthetase) ELISA Kit
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Product Name
Mouse IARS(Isoleucyl tRNA Synthetase) ELISA Kit
Alias
IARS ELISA Kit, Isoleucine--tRNA ligase ELISA Kit, IRS ELISA Kit, IleRS ELISA Kit, Isoleucyl-tRNA synthetase ELISA Kit, cytoplasmic ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IARS ELISA Kit allows for the in vitro quantitative determination of IARS concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.625-40ng/ml
Sensitivity
< 0.375ng/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IARS and the recovery rates were calculated by comparing the measured value to the expected amount of IARS in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-103 |
94 |
|
EDTA plasma(n=5) |
88-105 |
99 |
|
heparin plasma(n=5) |
87-105 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IARS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-103% |
86-103% |
85-104% |
|
EDTA plasma(n=5) |
84-96% |
84-94% |
84-100% |
|
heparin plasma(n=5) |
80-97% |
89-98% |
82-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
