FineTest
SKU:EM1026
Mouse FIX(Coagulation Factor IX) ELISA Kit
Mouse FIX(Coagulation Factor IX) ELISA Kit
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Product Name
Mouse FIX(Coagulation Factor IX) ELISA Kit
Alias
FIX ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
FIX ELISA Kit allows for the in vitro quantitative determination of FIX concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of FIX and the recovery rates were calculated by comparing the measured value to the expected amount of FIX in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-101 |
92 |
|
EDTA plasma(n=5) |
92-105 |
98 |
|
heparin plasma(n=5) |
85-98 |
90 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FIX and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-99% |
86-104% |
85-101% |
|
EDTA plasma(n=5) |
82-101% |
85-97% |
83-101% |
|
heparin plasma(n=5) |
80-100% |
81-87% |
84-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
