FineTest
SKU:EM0982
Mouse DGAT1(Diacylglycerol O-acyltransferase 1) ELISA Kit
Mouse DGAT1(Diacylglycerol O-acyltransferase 1) ELISA Kit
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Product Name
Mouse DGAT1(Diacylglycerol O-acyltransferase 1) ELISA Kit
Alias
hCG_24006 ELISA Kit, ARGP1 ELISA Kit, DGAT ELISA Kit, ACAT related gene product 1 ELISA Kit, acyl coenzyme A:cholesterol acyltransferase related gene 1 ELISA Kit, diacylglycerol O-acyltransferase 1 ELISA Kit, diglyceride acyltransferase ELISA Kit, diacylglycerol O-acyltransferase homolog 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
DGAT1 ELISA Kit allows for the in vitro quantitative determination of DGAT1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
18.75-1200pg/ml
Sensitivity
< 11.2pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of DGAT1 and the recovery rates were calculated by comparing the measured value to the expected amount of DGAT1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-100 |
92 |
|
EDTA plasma(n=5) |
88-101 |
95 |
|
heparin plasma(n=5) |
91-102 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DGAT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-105% |
85-101% |
90-105% |
|
EDTA plasma(n=5) |
82-101% |
84-99% |
84-93% |
|
heparin plasma(n=5) |
80-99% |
86-99% |
90-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
