FineTest
SKU:EM0914
Mouse CDKN1B(Cyclin Dependent Kinase Inhibitor 1B) ELISA Kit
Mouse CDKN1B(Cyclin Dependent Kinase Inhibitor 1B) ELISA Kit
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Product Name
Mouse CDKN1B(Cyclin Dependent Kinase Inhibitor 1B) ELISA Kit
Alias
CDKN1B ELISA Kit, Cyclin-dependent kinase inhibitor 1B ELISA Kit, Cyclin-dependent kinase inhibitor p27 ELISA Kit, p27Kip1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
CDKN1B ELISA Kit allows for the in vitro quantitative determination of CDKN1B concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
7.813-500pg/ml
Sensitivity
< 4.688pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of CDKN1B and the recovery rates were calculated by comparing the measured value to the expected amount of CDKN1B in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-97 |
94 |
|
EDTA plasma(n=5) |
90-103 |
95 |
|
heparin plasma(n=5) |
90-101 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CDKN1B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-101% |
90-104% |
85-101% |
|
EDTA plasma(n=5) |
83-99% |
84-101% |
84-97% |
|
heparin plasma(n=5) |
83-99% |
89-96% |
80-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
