FineTest
SKU:EM0903
Mouse CAST(Calpastatin) ELISA Kit
Mouse CAST(Calpastatin) ELISA Kit
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Product Name
Mouse CAST(Calpastatin) ELISA Kit
Alias
CAST ELISA Kit, Calpain inhibitor ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
CAST ELISA Kit allows for the in vitro quantitative determination of CAST concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of CAST and the recovery rates were calculated by comparing the measured value to the expected amount of CAST in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-103 |
98 |
|
EDTA plasma(n=5) |
95-104 |
98 |
|
heparin plasma(n=5) |
94-103 |
100 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CAST and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-98% |
98-104% |
89-98% |
|
EDTA plasma(n=5) |
87-100% |
85-93% |
85-99% |
|
heparin plasma(n=5) |
80-95% |
81-100% |
80-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
