FineTest
SKU:EM0884
Mouse C4a(Complement Component 4a) ELISA Kit
Mouse C4a(Complement Component 4a) ELISA Kit
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Product Name
Mouse C4a(Complement Component 4a) ELISA Kit
Alias
C4a ELISA Kit, C4A ELISA Kit, C4AD ELISA Kit, C4S ELISA Kit, CO4 ELISA Kit, CPAMD2 ELISA Kit, RG ELISA Kit, Complement Component 4a ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
C4a ELISA Kit allows for the in vitro quantitative determination of C4a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Mouse
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of C4a and the recovery rates were calculated by comparing the measured value to the expected amount of C4a in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-103 |
93 |
|
EDTA plasma(n=5) |
87-102 |
96 |
|
heparin plasma(n=5) |
87-102 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C4a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-99% |
89-104% |
92-105% |
|
EDTA plasma(n=5) |
82-95% |
87-101% |
82-94% |
|
heparin plasma(n=5) |
82-94% |
84-99% |
81-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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