FineTest
SKU:EM0858
Mouse AT-Ⅲ(anti-Thrombin Ⅲ) ELISA Kit
Mouse AT-Ⅲ(anti-Thrombin Ⅲ) ELISA Kit
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Product Name
Mouse AT-Ⅲ(anti-Thrombin Ⅲ) ELISA Kit
Alias
AT-Ⅲ ELISA Kit
Detection Method
Sandwich ELISA, Double Antigen
Application
AT-Ⅲ ELISA Kit allows for the in vitro quantitative determination of AT-Ⅲ concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.125-200ng/ml
Sensitivity
< 1.875ng/ml
Species
Mouse
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of AT-Ⅲ and the recovery rates were calculated by comparing the measured value to the expected amount of AT-Ⅲ in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
97-102 |
100 |
|
EDTA plasma(n=5) |
89-105 |
99 |
|
heparin plasma(n=5) |
86-104 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AT-Ⅲ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-105% |
91-103% |
92-105% |
|
EDTA plasma(n=5) |
84-99% |
87-101% |
85-99% |
|
heparin plasma(n=5) |
80-98% |
84-96% |
81-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
