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SKU:EM0731
Mouse Sirt3(NAD-dependent deacetylase sirtuin-3) ELISA Kit
Mouse Sirt3(NAD-dependent deacetylase sirtuin-3) ELISA Kit
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Product Name
Mouse Sirt3(NAD-dependent deacetylase sirtuin-3) ELISA Kit
Alias
Sirt3 ELISA Kit, SIR2L3 ELISA Kit, SIR2-like protein 3 ELISA Kit, SIRT3 ELISA Kit, EC 3.5.1 ELISA Kit, EC 3.5.1.- ELISA Kit, hSIRT3 ELISA Kit, mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase ELISA Kit, NAD-dependent deacetylase sirtuin-3 ELISA Kit, mitochondrial ELISA Kit, SIR2L3silent mating type information regulation 2 ELISA Kit, S.cerevisiae ELISA Kit, homolog 3 ELISA Kit, sir2-like 3 ELISA Kit, SIR2-like protein 3 ELISA Kit, sirtuin ELISA Kit, silent mating type information regulation 2 homolog 3 ELISA Kit, S. cerevisiae ELISA Kit, sirtuin ELISA Kit, silent mating type information regulation 2 ELISA Kit, S.cerevisiae ELISA Kit, homolog 3 ELISA Kit, sirtuin 3 ELISA Kit, sirtuin type 3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Sirt3 ELISA Kit allows for the in vitro quantitative determination of Sirt3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Mouse
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Sirt3 and the recovery rates were calculated by comparing the measured value to the expected amount of Sirt3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
91 |
|
EDTA plasma(n=5) |
85-100 |
95 |
|
heparin plasma(n=5) |
87-103 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sirt3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-101% |
93-105% |
88-104% |
|
EDTA plasma(n=5) |
86-97% |
84-98% |
83-91% |
|
heparin plasma(n=5) |
85-99% |
90-98% |
87-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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