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FineTest

SKU:EM0652

Mouse Pou5f1(POU domain, class 5, transcription factor 1) ELISA Kit

Mouse Pou5f1(POU domain, class 5, transcription factor 1) ELISA Kit

Regular price $450.00 USD
Regular price Sale price $450.00 USD
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size
Sensitivity
Range

Product Name

Mouse Pou5f1(POU domain, class 5, transcription factor 1) ELISA Kit

Alias

Pou5f1 ELISA Kit, Octamer-binding transcription factor 3 ELISA Kit, OTF-3 ELISA Kit, Octamer-binding protein 4 ELISA Kit, Oct4 ELISA Kit, Oct-4 ELISA Kit, NF-A3 ELISA Kit, Octamer-binding protein 3 ELISA Kit, Oct-3 ELISA Kit, Otf3g ELISA Kit, Otf4 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

Pou5f1 ELISA Kit allows for the in vitro quantitative determination of Pou5f1 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Species

Mouse

UniProt ID

P20263

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of Pou5f1 and the recovery rates were calculated by comparing the measured value to the expected amount of Pou5f1 in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

86-102

91

EDTA plasma(n=5)

85-103

96

heparin plasma(n=5)

85-96

90

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pou5f1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

88-96%

85-102%

89-103%

EDTA plasma(n=5)

88-101%

87-101%

83-101%

heparin plasma(n=5)

82-94%

84-94%

82-91%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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