FineTest
SKU:EM0649
Mouse Cfl1(Cofilin 1, Non-Muscle) ELISA Kit
Mouse Cfl1(Cofilin 1, Non-Muscle) ELISA Kit
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Product Name
Mouse Cfl1(Cofilin 1, Non-Muscle) ELISA Kit
Alias
Cfl1 ELISA Kit, 18 kDa phosphoprotein ELISA Kit, p18 ELISA Kit, Cofilin ELISA Kit, non-muscle isoform ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Cfl1 ELISA Kit allows for the in vitro quantitative determination of Cfl1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Mouse
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Cfl1 and the recovery rates were calculated by comparing the measured value to the expected amount of Cfl1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-96 |
91 |
|
EDTA plasma(n=5) |
85-101 |
96 |
|
heparin plasma(n=5) |
88-97 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cfl1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-105% |
87-104% |
85-101% |
|
EDTA plasma(n=5) |
85-99% |
91-99% |
82-99% |
|
heparin plasma(n=5) |
81-99% |
83-100% |
80-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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