FineTest
SKU:EM0643
Mouse H2-Aa(H-2 class II histocompatibility antigen, A-B alpha chain) ELISA Kit
Mouse H2-Aa(H-2 class II histocompatibility antigen, A-B alpha chain) ELISA Kit
Couldn't load pickup availability
Product Name
Mouse H2-Aa(H-2 class II histocompatibility antigen, A-B alpha chain) ELISA Kit
Alias
H2-Aa ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
H2-Aa ELISA Kit allows for the in vitro quantitative determination of H2-Aa concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.625-40ng/ml
Sensitivity
< 0.375ng/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of H2-Aa and the recovery rates were calculated by comparing the measured value to the expected amount of H2-Aa in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-103 |
95 |
|
EDTA plasma(n=5) |
88-102 |
94 |
|
heparin plasma(n=5) |
86-102 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of H2-Aa and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-102% |
86-104% |
87-105% |
|
EDTA plasma(n=5) |
88-100% |
84-98% |
84-101% |
|
heparin plasma(n=5) |
83-94% |
84-100% |
81-92% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
