FineTest
SKU:EM0637
Mouse H2afx(Histone H2A.x) ELISA Kit
Mouse H2afx(Histone H2A.x) ELISA Kit
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Product Name
Mouse H2afx(Histone H2A.x) ELISA Kit
Alias
H2afx ELISA Kit, H2a ELISA Kit, x ELISA Kit, H2AFX ELISA Kit, H2AX ELISA Kit, Histone H2A.X ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
H2afx ELISA Kit allows for the in vitro quantitative determination of H2afx concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of H2afx and the recovery rates were calculated by comparing the measured value to the expected amount of H2afx in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
94-103 |
99 |
|
EDTA plasma(n=5) |
90-104 |
97 |
|
heparin plasma(n=5) |
93-105 |
100 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of H2afx and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-102% |
86-103% |
91-105% |
|
EDTA plasma(n=5) |
84-98% |
82-90% |
84-99% |
|
heparin plasma(n=5) |
81-98% |
80-96% |
80-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
