FineTest
SKU:EM0527
Mouse Cenpb(Major centromere autoantigen B) ELISA Kit
Mouse Cenpb(Major centromere autoantigen B) ELISA Kit
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Product Name
Mouse Cenpb(Major centromere autoantigen B) ELISA Kit
Alias
Cenpb ELISA Kit, Major centromere autoantigen B ELISA Kit, Centromere protein B ELISA Kit, CENP-B ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Cenpb ELISA Kit allows for the in vitro quantitative determination of Cenpb concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Cenpb and the recovery rates were calculated by comparing the measured value to the expected amount of Cenpb in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
93-104 |
99 |
|
EDTA plasma(n=5) |
90-103 |
97 |
|
heparin plasma(n=5) |
92-99 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cenpb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
95-104% |
91-105% |
85-100% |
|
EDTA plasma(n=5) |
88-101% |
82-97% |
82-96% |
|
heparin plasma(n=5) |
83-98% |
84-99% |
82-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
