FineTest
SKU:EM0497
Mouse Wnt5a(Protein Wnt-5a) ELISA Kit
Mouse Wnt5a(Protein Wnt-5a) ELISA Kit
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Product Name
Mouse Wnt5a(Protein Wnt-5a) ELISA Kit
Alias
Wnt5a ELISA Kit, WNT5A ELISA Kit, Protein Wnt-5a ELISA Kit, wingless-type MMTV integRation site family ELISA Kit, member 5A ELISA Kit, WNT-5A protein ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Wnt5a ELISA Kit allows for the in vitro quantitative determination of Wnt5a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Wnt5a and the recovery rates were calculated by comparing the measured value to the expected amount of Wnt5a in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-102 |
94 |
|
EDTA plasma(n=5) |
87-102 |
91 |
|
heparin plasma(n=5) |
86-96 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wnt5a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-105% |
89-103% |
88-105% |
|
EDTA plasma(n=5) |
82-89% |
84-98% |
82-99% |
|
heparin plasma(n=5) |
83-95% |
85-100% |
86-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
