FineTest
SKU:EM0466
Mouse ACTH(Corticotropin) ELISA Kit
Mouse ACTH(Corticotropin) ELISA Kit
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Product Name
Mouse ACTH(Corticotropin Hormone) ELISA Kit
Alias
ACTH(Adrenocorticotropic Hormone)/Corticotropin/Adrenocorticotropin ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
ACTH ELISA Kit allows for the in vitro quantitative determination of ACTH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
12.5-800pg/ml
Sensitivity
< 7.5pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ACTH and the recovery rates were calculated by comparing the measured value to the expected amount of ACTH in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-99 |
96 |
|
EDTA plasma(n=5) |
89-103 |
97 |
|
heparin plasma(n=5) |
89-99 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-105% |
85-105% |
87-105% |
|
EDTA plasma(n=5) |
83-95% |
82-98% |
84-101% |
|
heparin plasma(n=5) |
81-97% |
84-96% |
91-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
