FineTest
SKU:EM0361
Mouse Hmgb4(High mobility group protein B4) ELISA Kit
Mouse Hmgb4(High mobility group protein B4) ELISA Kit
Couldn't load pickup availability
Product Name
Mouse Hmgb4(High mobility group protein B4) ELISA Kit
Alias
Hmgb4 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Hmgb4 ELISA Kit allows for the in vitro quantitative determination of Hmgb4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Hmgb4 and the recovery rates were calculated by comparing the measured value to the expected amount of Hmgb4 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-99 |
92 |
|
EDTA plasma(n=5) |
90-101 |
97 |
|
heparin plasma(n=5) |
85-97 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hmgb4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-102% |
87-105% |
90-96% |
|
EDTA plasma(n=5) |
88-99% |
82-97% |
82-99% |
|
heparin plasma(n=5) |
81-99% |
80-96% |
88-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
