FineTest
SKU:EM0346
Mouse Nucb2(Nucleobindin-2) ELISA Kit
Mouse Nucb2(Nucleobindin-2) ELISA Kit
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Product Name
Mouse Nucb2(Nucleobindin-2) ELISA Kit
Alias
Nucb2 ELISA Kit, NEFA ELISA Kit, Prepronesfatin ELISA Kit, DNA-binding protein NEFA ELISA Kit, Gastric cancer antigen Zg4 ELISA Kit, nucleobindin 2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Nucb2 ELISA Kit allows for the in vitro quantitative determination of Nucb2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Nucb2 and the recovery rates were calculated by comparing the measured value to the expected amount of Nucb2 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-100 |
92 |
|
EDTA plasma(n=5) |
89-105 |
99 |
|
heparin plasma(n=5) |
88-95 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nucb2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-96% |
89-105% |
90-102% |
|
EDTA plasma(n=5) |
83-98% |
83-99% |
84-100% |
|
heparin plasma(n=5) |
81-95% |
81-96% |
82-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
