FineTest
SKU:EM0335
Mouse Gfap(Glial fibrillary acidic protein) ELISA Kit
Mouse Gfap(Glial fibrillary acidic protein) ELISA Kit
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Product Name
Mouse Gfap(Glial fibrillary acidic protein) ELISA Kit
Alias
Gfap ELISA Kit, FLJ45472 ELISA Kit, glial fibrillary acidic protein ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Gfap ELISA Kit allows for the in vitro quantitative determination of Gfap concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Gfap and the recovery rates were calculated by comparing the measured value to the expected amount of Gfap in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
96 |
|
EDTA plasma(n=5) |
87-99 |
92 |
|
heparin plasma(n=5) |
93-104 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gfap and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-96% |
87-99% |
89-98% |
|
EDTA plasma(n=5) |
86-99% |
85-96% |
88-101% |
|
heparin plasma(n=5) |
87-99% |
81-87% |
83-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
