FineTest
SKU:EM0324
Mouse Alcam(CD166 antigen) ELISA Kit
Mouse Alcam(CD166 antigen) ELISA Kit
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Product Name
Mouse Alcam(CD166 antigen) ELISA Kit
Alias
ALCAM ELISA Kit, CD166 ELISA Kit, MEMD ELISA Kit, activated leucocyte cell adhesion molecule ELISA Kit, CD166 antigen ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Alcam ELISA Kit allows for the in vitro quantitative determination of Alcam concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Alcam and the recovery rates were calculated by comparing the measured value to the expected amount of Alcam in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-102 |
96 |
|
EDTA plasma(n=5) |
86-105 |
97 |
|
heparin plasma(n=5) |
87-100 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alcam and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-97% |
88-105% |
87-105% |
|
EDTA plasma(n=5) |
82-98% |
83-100% |
82-99% |
|
heparin plasma(n=5) |
82-97% |
81-97% |
83-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
