FineTest
SKU:EM0310
Mouse Hif1α(Hypoxia-inducible factor 1-alpha) ELISA Kit
Mouse Hif1α(Hypoxia-inducible factor 1-alpha) ELISA Kit
Couldn't load pickup availability
Product Name
Mouse Hif1α(Hypoxia-inducible factor 1-alpha) ELISA Kit
Alias
Hif1α ELISA Kit, HIF-1α ELISA Kit, HIF1A ELISA Kit, ARNT-interacting protein ELISA Kit, Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit, BHLHE78 ELISA Kit, Class E basic helix-loop-helix protein 78 ELISA Kit, HIF-1-alpha ELISA Kit, HIF1-alpha ELISA Kit, hypoxia-inducible factor 1-alpha ELISA Kit, Member of PAS protein 1 ELISA Kit, member of PAS superfamily 1 ELISA Kit, PAS domain-containing protein 8 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Hif1α ELISA Kit allows for the in vitro quantitative determination of Hif1α concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
125-8000pg/ml
Sensitivity
< 75pg/ml
Species
Mouse
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Hif1α and the recovery rates were calculated by comparing the measured value to the expected amount of Hif1α in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-103 |
96 |
|
EDTA plasma(n=5) |
88-100 |
96 |
|
heparin plasma(n=5) |
88-101 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hif1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-105% |
85-103% |
90-99% |
|
EDTA plasma(n=5) |
87-99% |
86-101% |
82-95% |
|
heparin plasma(n=5) |
81-98% |
80-100% |
80-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
Share
