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SKU:EM0310

Mouse Hif1α(Hypoxia-inducible factor 1-alpha) ELISA Kit

Mouse Hif1α(Hypoxia-inducible factor 1-alpha) ELISA Kit

Regular price $450.00 USD
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size
Sensitivity
Range

Product Name

Mouse Hif1α(Hypoxia-inducible factor 1-alpha) ELISA Kit

Alias

Hif1α ELISA Kit, HIF-1α ELISA Kit, HIF1A ELISA Kit, ARNT-interacting protein ELISA Kit, Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit, BHLHE78 ELISA Kit, Class E basic helix-loop-helix protein 78 ELISA Kit, HIF-1-alpha ELISA Kit, HIF1-alpha ELISA Kit, hypoxia-inducible factor 1-alpha ELISA Kit, Member of PAS protein 1 ELISA Kit, member of PAS superfamily 1 ELISA Kit, PAS domain-containing protein 8 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

Hif1α ELISA Kit allows for the in vitro quantitative determination of Hif1α concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

125-8000pg/ml

Sensitivity

< 75pg/ml

Species

Mouse

UniProt ID

Q61221

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of Hif1α and the recovery rates were calculated by comparing the measured value to the expected amount of Hif1α in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

88-103

96

EDTA plasma(n=5)

88-100

96

heparin plasma(n=5)

88-101

96

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hif1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

88-105%

85-103%

90-99%

EDTA plasma(n=5)

87-99%

86-101%

82-95%

heparin plasma(n=5)

81-98%

80-100%

80-98%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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