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SKU:EM0283
Mouse ACA-IgM(anti-Cardiolipin Antibody IgM) ELISA Kit
Mouse ACA-IgM(anti-Cardiolipin Antibody IgM) ELISA Kit
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Product Name
Mouse ACA-IgM(anti-Cardiolipin Antibody IgM) ELISA Kit
Alias
ACA-IgM ELISA Kit, anti-Cardiolipin Antibody IgM ELISA Kit
Detection Method
Sandwich ELISA, Double Antigen
Application
ACA-IgM ELISA Kit allows for the in vitro quantitative determination of ACA-IgM concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.125-200ng/ml
Sensitivity
< 1.875ng/ml
Species
Mouse
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ACA-IgM and the recovery rates were calculated by comparing the measured value to the expected amount of ACA-IgM in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-97 |
91 |
|
EDTA plasma(n=5) |
92-105 |
100 |
|
heparin plasma(n=5) |
89-105 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACA-IgM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-98% |
87-103% |
87-98% |
|
EDTA plasma(n=5) |
83-99% |
85-101% |
90-97% |
|
heparin plasma(n=5) |
83-99% |
87-100% |
81-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
