FineTest
SKU:EM0254
Mouse Fgf21(Fibroblast growth factor 21) ELISA Kit
Mouse Fgf21(Fibroblast growth factor 21) ELISA Kit
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Product Name
Mouse Fgf21(Fibroblast growth factor 21) ELISA Kit
Alias
FGF21 ELISA Kit, FGF-21 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Fgf21 ELISA Kit allows for the in vitro quantitative determination of Fgf21 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Fgf21 and the recovery rates were calculated by comparing the measured value to the expected amount of Fgf21 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-103 |
97 |
|
EDTA plasma(n=5) |
85-105 |
97 |
|
heparin plasma(n=5) |
88-104 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fgf21 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-105% |
85-99% |
89-104% |
|
EDTA plasma(n=5) |
83-101% |
83-98% |
82-98% |
|
heparin plasma(n=5) |
80-99% |
81-95% |
90-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
