FineTest
SKU:EM0227
Mouse Cntf(Ciliary neurotrophic factor) ELISA Kit
Mouse Cntf(Ciliary neurotrophic factor) ELISA Kit
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Product Name
Mouse Cntf(Ciliary neurotrophic factor) ELISA Kit
Alias
Cntf ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Cntf ELISA Kit allows for the in vitro quantitative determination of Cntf concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
7.813-500pg/ml
Sensitivity
< 4.688pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Cntf and the recovery rates were calculated by comparing the measured value to the expected amount of Cntf in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-102 |
94 |
|
EDTA plasma(n=5) |
88-101 |
95 |
|
heparin plasma(n=5) |
85-103 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cntf and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-102% |
86-103% |
85-100% |
|
EDTA plasma(n=5) |
82-89% |
84-99% |
89-100% |
|
heparin plasma(n=5) |
80-99% |
81-97% |
83-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
