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SKU:EM0183

Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Regular price $400.00 USD
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size
Sensitivity
Range

Product Name

Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Alias

TNFα ELISA KitTumor Necrosis Factor Alpha ELISA KitTNF-α ELISA KitDIF ELISA KitTNF-alpha ELISA KitTNFA ELISA KitTNFSF2 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

TNF-α ELISA Kit allows for the in vitro quantitative determination of TNFα concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

3.906-250pg/ml

Sensitivity

< 2.344pg/ml

Species

Mouse

UniProt ID

P06804

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TNFα and the recovery rates were calculated by comparing the measured value to the expected amount of TNFα in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

85-105

96

EDTA plasma(n=5)

85-105

96

heparin plasma(n=5)

85-105

91

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

90-105%

86-100%

93-105%

EDTA plasma(n=5)

84-99%

96-100%

82-100%

heparin plasma(n=5)

81-100%

82-98%

81-100%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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