FineTest
SKU:EM0109
Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
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Product Name
Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
Alias
IL-1β ELISA Kit, Interleukin 1 Beta ELISA Kit, IL-1beta ELISA Kit, IL1B ELISA Kit, IL1-BETA ELISA Kit, IL1F2 ELISA Kit, catabolin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-1β ELISA Kit allows for the in vitro quantitative determination of IL-1b concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
12.5-800pg/ml
Sensitivity
< 7.5pg/ml
Species
Mouse
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-1b and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1b in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-100 |
92 |
|
EDTA plasma(n=5) |
87-94 |
92 |
|
heparin plasma(n=5) |
90-105 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-99% |
85-95% |
85-100% |
|
EDTA plasma(n=5) |
91-99% |
89-99% |
88-101% |
|
heparin plasma(n=5) |
80-96% |
80-91% |
81-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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