FineTest
SKU:EM0093
Mouse IFN-γ(Interferon Gamma) ELISA Kit
Mouse IFN-γ(Interferon Gamma) ELISA Kit
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Product Name
Mouse IFN-γ(Interferon Gamma) ELISA Kit
Alias
IFN-γ ELISA Kit, Interferon Gamma ELISA Kit, IFNG ELISA Kit, IFG ELISA Kit, IFI ELISA Kit, Type II Interferon ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IFN-γ ELISA Kit allows for the in vitro quantitative determination of IFNγ concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IFNγ and the recovery rates were calculated by comparing the measured value to the expected amount of IFNγ in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
96-101 |
99 |
|
EDTA plasma(n=5) |
91-100 |
97 |
|
heparin plasma(n=5) |
91-104 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFNγ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-101% |
95-103% |
85-101% |
|
EDTA plasma(n=5) |
82-101% |
92-101% |
86-101% |
|
heparin plasma(n=5) |
86-96% |
81-93% |
81-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
