FineTest
SKU:EM0019
Mouse BAFF/CD257(B-cell Activating Factor) ELISA Kit
Mouse BAFF/CD257(B-cell Activating Factor) ELISA Kit
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Product Name
Mouse BAFF/CD257(B-cell Activating Factor) ELISA Kit
Alias
BAFF ELISA Kit, CD257 ELISA Kit, TNFSF13B ELISA Kit, BLYS ELISA Kit, TALL1 ELISA Kit, THANK ELISA Kit, TNFSF20 ELISA Kit, ZTNF4 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
BAFF/CD257 ELISA Kit allows for the in vitro quantitative determination of BAFF/CD257 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
62.5-4000pg/ml
Sensitivity
< 37.5pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of BAFF/CD257 and the recovery rates were calculated by comparing the measured value to the expected amount of BAFF/CD257 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-96 |
91 |
|
EDTA plasma(n=5) |
87-104 |
96 |
|
heparin plasma(n=5) |
87-105 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BAFF/CD257 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-102% |
88-105% |
90-102% |
|
EDTA plasma(n=5) |
87-97% |
84-98% |
85-101% |
|
heparin plasma(n=5) |
81-92% |
88-96% |
82-91% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
