FineTest
SKU:EH4124
Human Cytochrome b-245 Beta Polypeptide ELISA Kit
Human Cytochrome b-245 Beta Polypeptide ELISA Kit
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Product Name
Human Cytochrome b-245 Beta Polypeptide ELISA Kit
Alias
NOX2 ELISA Kit, CGD91-phox ELISA Kit, NADPH oxidase 2 ELISA Kit, gp91phox ELISA Kit, p22 phagocyte B-cytochrome ELISA Kit, gp91-1 ELISA Kit, Superoxide-geneRating NADPH oxidase heavy chain subunit ELISA Kit, Neutrophil cytochrome b 91 kDa polypeptide ELISA Kit, Heme-binding membrane glycoprotein gp91phox ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Cytochrome b-245 Beta Polypeptide ELISA Kit allows for the in vitro quantitative determination of CYBB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of CYBB and the recovery rates were calculated by comparing the measured value to the expected amount of CYBB in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-97 |
90 |
|
EDTA plasma(n=5) |
90-105 |
99 |
|
heparin plasma(n=5) |
88-104 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYBB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
95-105% |
92-105% |
86-99% |
|
EDTA plasma(n=5) |
86-99% |
82-94% |
82-100% |
|
heparin plasma(n=5) |
80-100% |
87-96% |
85-92% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
