FineTest
SKU:EH4017
Human β-actin(Beta actin) ELISA Kit
Human β-actin(Beta actin) ELISA Kit
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Product Name
Human β-actin(Beta actin) ELISA Kit
Alias
β-actin ELISA Kit, ACTB ELISA Kit, actin ELISA Kit, beta ELISA Kit, actin ELISA Kit, cytoplasmic 1 ELISA Kit, beta cytoskeletal actin ELISA Kit, Beta-actin ELISA Kit, PS1TP5-binding protein 1 ELISA Kit, PS1TP5BP1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
β-actin ELISA Kit allows for the in vitro quantitative determination of β-actin concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of β-actin and the recovery rates were calculated by comparing the measured value to the expected amount of β-actin in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-103 |
93 |
|
EDTA plasma(n=5) |
85-97 |
91 |
|
heparin plasma(n=5) |
85-103 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of β-actin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-97% |
98-104% |
85-102% |
|
EDTA plasma(n=5) |
96-100% |
85-94% |
85-98% |
|
heparin plasma(n=5) |
80-100% |
81-99% |
80-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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