FineTest
SKU:EH4004
Human CHAT(Choline O-acetyltransferase) ELISA Kit
Human CHAT(Choline O-acetyltransferase) ELISA Kit
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Product Name
Human CHAT(Choline O-acetyltransferase) ELISA Kit
Alias
ChAT ELISA Kit, CHOACTase ELISA Kit, Choline acetylase ELISA Kit, Choline O-acetyltransferase ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
CHAT ELISA Kit allows for the in vitro quantitative determination of CHAT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of CHAT and the recovery rates were calculated by comparing the measured value to the expected amount of CHAT in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
96 |
|
EDTA plasma(n=5) |
87-105 |
95 |
|
heparin plasma(n=5) |
91-103 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CHAT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
96-103% |
86-101% |
88-100% |
|
EDTA plasma(n=5) |
85-97% |
82-100% |
82-100% |
|
heparin plasma(n=5) |
85-99% |
83-98% |
80-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
