FineTest
SKU:EH4005
Human 5-hydroxytryptamine ELISA Kit
Human 5-hydroxytryptamine ELISA Kit
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Product Name
Human 5-hydroxytryptamine ELISA Kit
Alias
ST ELISA Kit, 5-HT ELISA Kit, Serotonin ELISA Kit, 5-Hydroxytryptamine ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
5-hydroxytryptamine ELISA Kit allows for the in vitro quantitative determination of 5-hydroxytryptamine concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100ng/ml
Sensitivity
< 0.938ng/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of 5-hydroxytryptamine and the recovery rates were calculated by comparing the measured value to the expected amount of 5-hydroxytryptamine in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
93-104 |
97 |
|
EDTA plasma(n=5) |
87-105 |
95 |
|
heparin plasma(n=5) |
89-102 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-hydroxytryptamine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
92-103% |
94-105% |
88-98% |
|
EDTA plasma(n=5) |
83-99% |
87-101% |
86-96% |
|
heparin plasma(n=5) |
83-91% |
90-95% |
84-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
