FineTest
SKU:EH3931
Human TWEAK(Tumour Necrosis Factor Related Weak Inducer of Apoptosis) ELISA Kit
Human TWEAK(Tumour Necrosis Factor Related Weak Inducer of Apoptosis) ELISA Kit
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Product Name
Human TWEAK(Tumour Necrosis Factor Related Weak Inducer of Apoptosis) ELISA Kit
Alias
TWEAK/TNFSF12/APO3 ligand/APO3LMGC20669/DR3LGTWEAKAPO3/DR3 ligand/MGC129581/TNF-related weak inducer of apoptosis/tumor necrosis factor(ligand) superfamily, member 12/tumor necrosis factor ligand superfamily member 12 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TWEAK ELISA Kit allows for the in vitro quantitative determination of TWEAK concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.2-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TWEAK and the recovery rates were calculated by comparing the measured value to the expected amount of TWEAK in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-100 |
94 |
|
EDTA plasma(n=5) |
86-104 |
92 |
|
heparin plasma(n=5) |
87-97 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TWEAK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-100% |
87-105% |
90-103% |
|
EDTA plasma(n=5) |
90-96% |
86-95% |
85-91% |
|
heparin plasma(n=5) |
90-100% |
93-99% |
88-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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