FineTest
SKU:EH3898
Human Total P53(Tumor Protein p53) ELISA Kit
Human Total P53(Tumor Protein p53) ELISA Kit
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Product Name
Human Total P53(Tumor Protein p53) ELISA Kit
Alias
TP53 ELISA Kit, BCC7 ELISA Kit, LFS1 ELISA Kit, TRP53 ELISA Kit, Antigen NY-CO-13 ELISA Kit, FLJ92943 ELISA Kit, LFS1TRP53 ELISA Kit, p53 ELISA Kit, p53 tumor suppressor ELISA Kit, P53cellular tumor antigen p53 ELISA Kit, Phosphoprotein p53 ELISA Kit, transformation-related protein 53 ELISA Kit, tumor protein p53 ELISA Kit, Tumor suppressor p53 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Total P53 ELISA Kit allows for the in vitro quantitative determination of TP53 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TP53 and the recovery rates were calculated by comparing the measured value to the expected amount of TP53 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-102 |
93 |
|
EDTA plasma(n=5) |
85-103 |
94 |
|
heparin plasma(n=5) |
85-101 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TP53 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-102% |
90-104% |
91-100% |
|
EDTA plasma(n=5) |
84-101% |
84-98% |
91-99% |
|
heparin plasma(n=5) |
85-98% |
84-98% |
80-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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