FineTest
SKU:EH3791
Human Smad4/MADH4(Mothers Against Decapentaplegic Homolog 4) ELISA Kit
Human Smad4/MADH4(Mothers Against Decapentaplegic Homolog 4) ELISA Kit
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Product Name
Human Smad4/MADH4(Mothers Against Decapentaplegic Homolog 4) ELISA Kit
Alias
Smad4 ELISA Kit, MADH4 ELISA Kit, MAD homolog 4 ELISA Kit, Mothers against DPP homolog 4 ELISA Kit, Deletion target in pancreatic carcinoma 4 ELISA Kit, SMAD family member 4 ELISA Kit, SMAD 4 ELISA Kit, Smad4 ELISA Kit, Hsmad4 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Smad4/MADH4 ELISA Kit allows for the in vitro quantitative determination of Smad4/MADH4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Smad4/MADH4 and the recovery rates were calculated by comparing the measured value to the expected amount of Smad4/MADH4 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-102 |
97 |
|
EDTA plasma(n=5) |
87-98 |
92 |
|
heparin plasma(n=5) |
86-99 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Smad4/MADH4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
94-105% |
86-105% |
90-105% |
|
EDTA plasma(n=5) |
82-94% |
82-99% |
87-100% |
|
heparin plasma(n=5) |
85-99% |
81-95% |
85-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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