FineTest
SKU:EH3788
Human SM/SPH(Sphingomyelin) ELISA Kit
Human SM/SPH(Sphingomyelin) ELISA Kit
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Product Name
Human SM/SPH(Sphingomyelin) ELISA Kit
Alias
SM ELISA Kit, SPH ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
SM/SPH ELISA Kit allows for the in vitro quantitative determination of SM/SPH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.125-200ng/ml
Sensitivity
< 1.875ng/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SM/SPH and the recovery rates were calculated by comparing the measured value to the expected amount of SM/SPH in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-101 |
93 |
|
EDTA plasma(n=5) |
92-104 |
97 |
|
heparin plasma(n=5) |
89-101 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SM/SPH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-104% |
89-103% |
87-99% |
|
EDTA plasma(n=5) |
86-98% |
85-98% |
84-101% |
|
heparin plasma(n=5) |
84-97% |
82-99% |
82-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
