FineTest
SKU:EH3776
Human SIAE(Sialic Acid Acetylesterase) ELISA Kit
Human SIAE(Sialic Acid Acetylesterase) ELISA Kit
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Product Name
Human SIAE(Sialic Acid Acetylesterase) ELISA Kit
Alias
SIAE ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SIAE ELISA Kit allows for the in vitro quantitative determination of SIAE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SIAE and the recovery rates were calculated by comparing the measured value to the expected amount of SIAE in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-105 |
94 |
|
EDTA plasma(n=5) |
86-104 |
95 |
|
heparin plasma(n=5) |
85-104 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIAE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-103% |
88-104% |
86-103% |
|
EDTA plasma(n=5) |
90-99% |
82-99% |
82-97% |
|
heparin plasma(n=5) |
83-99% |
80-85% |
83-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
